Arabidopsis Disulfide Reductase, Trx-h2, Functions as an RNA Chaperone under Cold Stress

The thioredoxin-h (Trx-h) family of Arabidopsis thaliana comprises cytosolic disulfide reductases. However, the physiological function Trx-h2, which contains an additional 19 amino acids at its N-terminus, remains unclear. In this study, we investigated molecular Trx-h2 both in vitro and vivo found that overexpression (Trx-h2OE) lines showed significantly longer roots than wild-type plants under cold stress. Therefore, further role Our results revealed functions as RNA chaperone by melting misfolded non-functional RNAs, facilitating their correct folding into active forms with native conformation. We binds to efficiently melts nucleic (ssDNA, dsDNA, RNA), facilitates export mRNAs from nucleus cytoplasm Moreover, increased survival rate cold-sensitive E. coli BX04 cells low temperature. Thus, our data show performs stress, thus increasing plant tolerance.